منابع مشابه
Multi-photon excitation microscopy
Multi-photon excitation (MPE) microscopy plays a growing role among microscopical techniques utilized for studying biological matter. In conjunction with confocal microscopy it can be considered the imaging workhorse of life science laboratories. Its roots can be found in a fundamental work written by Maria Goeppert Mayer more than 70 years ago. Nowadays, 2PE and MPE microscopes are expected to...
متن کاملTwo-photon excitation STED microscopy.
We report sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy achieved by merging this technique with stimulated-emission depletion (STED). We demonstrate an easy-to-implement and promising laser combination based on a short-pulse laser source for two-photon excitation and a continuous-wave (CW) laser source for resolution enhancement. Images of fluorescent nanopar...
متن کاملTwo-photon excitation fluorescence microscopy.
Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced speci...
متن کامل4pi-microscopy with 1-photon Excitation
Over the last years two-photon excitation 4Pi-microscopy with an axial resolution of ~100 nm has been demonstrated in multiple biological applications [1-3]. The artefact-free presentation of the recorded structures has been possible because of the contiguous optical transfer function (OTF), which allows reliable image deconvolution. The use of two-photon excitation (2PE) has been critical to s...
متن کاملConfocal microscopy and multi-photon excitation microscopy of human skin in vivo.
This paper compares tandem scanning reflected light confocal microscopy and multi-photon excitation microscopy for the observation of human skin in vivo. Tandem scanning confocal light microscopy based on a white light source can provide video-rate image acquisition from the skin surface to the epidermal-dermal junction. Multi-photon excitation is induced by a 80 MHz pulse train of femtosecond ...
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ژورنال
عنوان ژورنال: BioMedical Engineering OnLine
سال: 2006
ISSN: 1475-925X
DOI: 10.1186/1475-925x-5-36